Hb estimation methods

Discussion in 'Question Zone' started by David., Dec 27, 2007.

  1. David.

    David. Guest

    Hb estimation methods include all except:
    a) Drabkins method
    b) Wintrobes method
    c) Sahli’s method
    d) Spectrophotometry
  2. David.

    David. Guest

    Ans. b) Wintrobes method (used for ESR)
    Drabkin's solution containing potassium ferricyanide, potassium cyanide and sodium bicarbonate was prepared. 20 microliter of blood was added to 5ml of Drabkin's solution. Readings were taken at 530 nm in a spectrophotometer. Haemoglobin values were calculated from a haemoglobin curve prepared using haemoglobin standard.

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